p70s6 kinase 49d7  (Cell Signaling Technology Inc)

Journal: The EMBO Journal

Article Title: Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis

doi: 10.1038/s44318-024-00359-z

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-p70 S6 Kinase (49D7) , CST, USA , Cat #2708.

Techniques: Recombinant, Sequencing, Modification, Membrane, Lysis, Transfection, Magnetic Beads, Software, Cytometry, In Vitro, cDNA Synthesis, Plasmid Preparation, Isolation, Mutagenesis, Silver Staining

Journal: Scientific reports

Article Title: TSC complex decrease the expression of mTOR by regulated miR-199b-3p.

doi: 10.1038/s41598-025-85706-8

Figure Lengend Snippet: Fig. 4. miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein. (H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR- 199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.

Article Snippet: Cat.No.#4308), mouse IgG (Stanta Cruz, Cat.No.sc-2025), mouse anti Myc-tag monoclonal antibody (ABclonal, Cat.No.AE010), DDDDK tag (flag) mouse monoclonal antibody (Proteintech, Cat.No.66008-3Ig), mTOR (phosphor-S2448) polyclonal antibody (Bioworld, Cat.No.BS4706), Beta actin Polyclonal antibody (Absin, Cat.No.abs132001), 4E-BP1 (53H11) rabbit monoclonal antibody (Cell Signaling, Cat.No.#9644), Phospho-4E-BP1 rabbit monoclonal antibody (Thr37/46) (236B4) (Cell Signaling, Cat.No.#2855), p70S6 Kinase (49D7) rabbit monoclonal antibody (Cell Signaling, Cat. No. #2708), Phospho-p70S6 Kinase rabbit monoclonal antibody (Ser371) (Cell Signaling, Cat. No. #9208), goat anti-rabbit IgG (H + L), AKT mouse monoclonal antibody (Santa cruz, Cat.No.sc-5298), p-AKT mouse monoclonal antibody(Ser 473)( Santa cruz, Cat.No.sc-293125).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Sequencing, Luciferase, Reporter Assay, Control

Journal: Cell Reports Medicine

Article Title: CD97 maintains tumorigenicity of glioblastoma stem cells via mTORC2 signaling and is targeted by CAR Th9 cells

doi: 10.1016/j.xcrm.2024.101844

Figure Lengend Snippet:

Article Snippet: p70 S6 Kinase (49D7) Rabbit mAb , Cell Signaling Technology , Cat# 2708; RRID: AB_390722.

Techniques: Produced, Virus, Plasmid Preparation, Recombinant, Purification, Cell Culture, Cell Isolation, Reporter Gene Assay, cDNA Synthesis, Apoptosis Assay, Cytotoxicity Assay, Gene Expression, shRNA, Sequencing, Amplification, Software, Microscopy, Western Blot

Journal: The EMBO Journal

Article Title: Calcium signaling from damaged lysosomes induces cytoprotective stress granules

doi: 10.1038/s44318-024-00292-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit p70 S6 Kinase (49D7) , Cell Signaling Technology , #2708.

Techniques: Recombinant, Sequencing, Mutagenesis, Control, CRISPR, Staining, Magnetic Beads, Transfection, Lysis, Cytotoxicity Assay, Protease Inhibitor, Software